In the second half of the 21st century, the effects of climate change associated with population growth, aggressive urbanisation and the resulting deterioration of the natural environment have become particularly noticeable. In terms of modern construction and sustainable design, it is valuable to subject a building to a life cycle analysis (LCA), which covers the investment process from design to production of materials, erection of the building, operation and potential demolition. Wood, a natural raw material present in architecture for centuries, unites the ecological, economic and social principles of sustainable design. Thanks to innovative processing techniques such as cross-laminated timber (CLT) technology, wood can meet the challenges of modernity. Given the growing consumer interest in pro-environmental attitudes and the tightening of European Union regulations, the main emphasis will likely be on selecting environmentally friendly materials with a low carbon footprint, favourable energy balance, and ultimately, recovery potential. Based on this hypothesis, the paper presents a comparative LCA analysis of use CLT and concrete technology for the construction of an educational building at the Marszewo Forest Botanical Garden in Gdynia.