The non-uniformity of temperature field distribution of long-span steel structure is proportional to the intensity of solar radiation. Based on the background of Guangzhou Baiyun Station large-span complex steel roof structure, this paper studies the non-uniformtemperature field distribution of large-span steel structure under the Summer Solstice daily radiation-thermal-fluid coupling action based on Star-ccm¸ finite element software, and uses Spa2000 software to analyze the stress and deformation of steel roof under temperature action. Combined with the on-site temperature monitoring, the maximum difference with the measured value is 2.5˚C compared with the numerical simulation results, which verifies the validity of the finite element simulation. The results show that: from 8:00, with the increase of solar altitude angle, the intensity of solar radiation increases, the temperature rises, and the temperature distribution of large-span steel structure becomes more and more non-uniform. From14:00 to18:00, the solar radiation weakens, and the temperature distribution tends to be uniform. Finally, reasonable construction suggestions and measures are proposed to reduce the adverse effects of temperature effects, which can provide theoretical references for the safe construction and normal operation of large-span steel structures located in the subtropics.