The dynamic analyses are of key importance in the cognitive process in terms of the correct operation of structures loaded with time alternating forces. The development of vehicle industry, which directly results in an increase in the speed of moving vehicles, forces the design of engineering structures that ensure their safe use. The authors of the paper verified the influence of speed and vehicle parameters such as mass, width of track of wheels and their number on the values of displacements and accelerations of selected bridge elements. The problem was treated as the case study, because the analyses were made for one bridge and the passage of three types of locomotives. The response of the structure depends on the technological solutions adopted in the bridge, its technical condition, as well as the quotient of the length of the object and vehicle. A new bridge structure was analyzed and dynamic tests were carried out for trainsets consisting of one and two locomotives. During the actual dynamic tests, the structure was loaded with a locomotive moving at a maximum speed of 160 km/h.