In this paper, based on the feasible method and sensors for the full-scale prestressed monitor, the novel optical fiber sensors and the traditional monitoring sensors will be set up into two prestressed concrete beams with the same geometrical dimensions, material properties, and construction conditions, etc. to investigate the working state of the novel sensors and obtain the evolution law of prestress loss of the prestressed feature component under the static load. The results show that the evolution law of prestress loss of the loaded beam under the condition of no damage state and initial crack is the same as the non-loaded one; however, the prestress loss increases with the increase of time under the situation with the limit crack. The total loss of the prestressed beam with the limit crack is 36.4% without damage. The prestress loss of the prestressed beam under the static load increase with the development of the crack (injury).